METHODS: The expression of MMP-2 in of the pulp of the healthy and carious tooth were examined by immunohistochemical staining, RT-PCR, real-time PCR and western-blot. 以健康、浅龋和深龋牙齿中牙髓组织为研究对象,利用免疫组化、RT-PCR、实时荧光定量PCR和Western印迹检测MMP-2在其中的表达差异。
Methods Apply force on right maxillary first molars in rats, and examine the decalcified alveolodental connected tissue in different stage of tooth movement with immunohistochemical staining technique ( SP method). 方法对大鼠正畸牙移动不同阶段的牙及牙周组织联合切片,进行免疫组织化学研究。